Typing of nontypable staphylococci by lysogeny.
نویسندگان
چکیده
Strains of coagulase-positive staphylococci which were nontypable with the routine typing set of phages could be typed by lysogeny with phage-propagating strains as indicators and with ultraviolet induction. About 10% of the strains could be typed without induction. About 36% of them could be typed by this method when ultraviolet irradiation was used as an inducing agent. The phage groups from which the majority of the nontypable staphylococci originated were easily identified by this method of typing.
منابع مشابه
Bacteriophage Typing of the Staphylococci.
INTRODUCTION ...................................................................... 253 TYPING PHAGES...................................................................... 255 TECHNIQUES OF PHAGE PROPAGATION ........................................................... 257 STERILIZATION AND PRESERVATION OF LYSATES ................................................. 258 TECHNIQUE OF PHAGE TYPING.......
متن کاملLysogeny in staphylococci.
Blair, John E. (Hospital for Joint Diseases, New York, N. Y.) and Miriam Carr. Lysogeny in staphylococci. J. Bacteriol. 82:984-993. 1961.-Changes in the phage typing patterns of strains of staphylococci of the 80/81-52/52A/80/81 complex and of phage group III were produced by lysogenization with temperate phages derived from selected strains of Staphylococcus aureus. The phages used were of the...
متن کاملStudies on lysogeny in staphylococci.
A staphylococcus typing phage (NCTC 8426) was found to form lysogenic systems with three strains of staphylococci. In the case of two of these strains, staphylococci lysogenically infected with the typing phage were now lysed by the temperate phages they had previously carried. This loss of immunity was due to the replacement of the originally carried prophage by the prophage of phage 8426. Thi...
متن کاملComparison of heat shocking and acridine orange treatment in phage typing of nontypable strains of Staphylococcus aureus.
Forty-five percent of 108 nontypable strains of Staphylococcus aureus from clinical and environmental sources were phage typable after heat shock and acridine orange treatment. Although phage patterns were produced by different types of treatment, the same phage groups were present, showing specificity. The newly typable strains appear to be similar in phage group distribution to the primary ty...
متن کاملExperiences and observations with the typing of Staphylococcus aureus phage 94.
During a 28-month period, 5,078 cultures from a variety of anatomical sites were received for staphylococcal phage typing. Of these, 503 (10%) were not suitable for the procedure requested. Of the 4,575 viable cultures, 1,030 (23%) of the microorganisms were nontypable at both the routine test dilution (RTD) and at 100 times RTD. Of the 3,545 typable organisms, 3,061 (86%) were lysed at RTD, wh...
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ورودعنوان ژورنال:
- Applied microbiology
دوره 15 1 شماره
صفحات -
تاریخ انتشار 1967